CALL FOR PAPERS Integrative and Translational Physiology: Inflammation and Immunity in Organ System Physiology Defining the differential sensitivity to norepinephrine and angiotensin II in the ovine uterine vasculature

Rosenfeld CR, DeSpain K, Liu X-t. Defining the differential sensitivity to norepinephrine and angiotensin II in the ovine uterine vasculature. Am J Physiol Regul Integr Comp Physiol 302: R59–R67, 2012. First published October 26, 2011; doi:10.1152/ajpregu.00424.2011.—The intact ovine uterine vascular bed (UVB) is sensitive to -agonists and refractory to angiotensin II (ANG II) during pregnancy; the converse occurs in the systemic circulation. The mechanism(s) responsible for these differences in uterine sensitivity are unclear and may reflect predominance of nonconstricting AT2 receptors (AT2R) in uterine vascular smooth muscle (UVSM). The contribution of the placental vasculature also is unclear. Third generation and precaruncular/placental arteries from nonpregnant (n 16) and term pregnant (n 23) sheep were used to study contraction responses to KCl, norepinephrine (NE), and ANG II (with/without ATR specific inhibitors) and determine UVSM ATR subtype expression and contractile protein content. KCl and NE increased third generation and precaruncular/ placental UVSM contractions in a doseand pregnancy-dependent manner (P 0.001). ANG II only elicited modest contractions in third generation pregnant UVSM (P 0.04) and none in precaruncular/placental UVSM. Moreover, compared with KCl and NE, ANG II contractions were diminished 5-fold. Whereas KCl and ANG II contracted third generation precaruncular/placental UVSM, NEinduced contractions were similar throughout the UVB. However, each agonist increased third generation contractions 2-fold at term, paralleling increased actin/myosin and cellular protein content (P 0.01). UVSM AT1R and AT2R expression was similar throughout the UVB and unchanged during pregnancy (P 0.1). AT1R inhibition blocked ANG II-mediated contractions; AT2R blockade, however, did not enhance contractions. AT2R predominate throughout the UVB of nonpregnant and pregnant sheep, contributing to an inherent refractoriness to ANG II. In contrast, NE elicits enhanced contractility throughout the ovine UVB that exceeds ANG II and increases further at term pregnancy.